The Center of Protein Designist

HELP

ver 1.00-2.31(code name:Mountain dragon)

Unpack a downloaded zip-file.

If you have gotten Java Runtime Environment(JRE), Double-click the "ProteinDesignist.jar"(windows)

Make original proteins

1. Insert your sequence.
2. Push the Create button.
   • Make the original protein.
   • Calculate the amino acid composition, chain length, and molecular weight.
3. Then, if you select the amino acid, you can modificate its amino acid.
4. You can digest the created protein, too.
5. ...... etc.

Polysaccharide Maker (You can modify original suger chain at "Serine(S)", "Threonine(T)", and "Asparagine(N)". after ver2.00

1. Select the amino acid(S, T, or N).
2. Polysaccharide Maker start.
   • Push the "Start/Renew" button.
   • Select a monosaccharide.
   • Select a binding type.
3. Then, you can make branch.
4. Push the "Finish" button.
5. You will return the protein designing.
6. You can save the suger data "*.psm".

Modifications

1. N-teminal methylation
2. N-teminal acetylation
3. Phosphorylation (S and T)
4. Phosphorylation (Y)
5. Disulfide
6. Acetylation (K)
7. C-teminal amidation
8. N-binding type Glycosilation(N)
9. O-binding type Glycosilation(S and T)

Sample picture

Idea, demands, and new sequence entries for ProteinDesignist are "".