<HTML>

<HEAD>

<TITLE>Protein dynamics project for multicomponents</TITLE>

</HEAD>

<BODY>

<!--copyright Tatsuya Yamamoto--> 

<p style="text-align: right;"><A href="http://homepage1.bb-west.ne.jp/pepdesi/topJ.html">Japanese</A> <A href="http://homepage1.bb-west.ne.jp/pepdesi/index.html">TOP page</A></p>

<HR>

<font size="7">Protein dynamics project for multicomponents</font>

<HR>


<table border="0">

<tr>

  <td><IMG SRC="kouzo.bmp" ALT="kouzo"></td>

  <td>We analyze protein dynamics in multicomponent system, using mass spectrometry and H/D exchange.

　　　<ul>

  		<li>Ribosome dynamics</li>

  		<li>Structural dynamics of other multicomponent systems</li>

		<li>Protein dynamics for Medical study</li>

  		<li>Development of new tool, software, and experiments for protein dynamics</li>

	</ul>

  </td>

</tr>

</table><tr>

    <dl><dt><font size="6">R</font>ibosome dynamics</dt><a href="reference.html#P4">[P4]</a>,<a href="reference.html#P6">[P6]</a>,<a href="reference.html#P7">[P7]</a>,<a href="reference.html#RJ2">[Rj2]</a>,<a href="reference.html#RJ3">[Rj3]</a>

<br>

    <dd>70S ribosome is the macro-complex (MW 2.3 MDa) composed of 55 proteins and three rRNAs.<br>X-ray crystallography and cryo-electron microscopy succeed the movement by comparing static structures, however it is difficult to detect the dynamics of each protein in solution because of the molecular size. In 2006, We reported the new approach using mass spectrometry and H/D exchange, which showed information of dynamics of their 52 proteins on a spectrum.       

    </dd></dl>

    <dl><dt><font size="6">S</font>tructural dynamics of other multicomponent systems</dt><a href="reference.html#P4">[P4]</a><br>

    <dd><li>Biology and structural dynamics of protein complexes binding with gas molecules</li>

    <li>Biology and structural dynamics(assembly) of membrane protein complexes</li>    

    </dd></dl>

    <dl><dt><font size="6">P</font>rotein dynamics for Medical study</dt><a href="reference.html#P1">[P1]</a>,<a href="reference.html#P2">[P2]</a>,<a href="reference.html#P3">[P3]</a>,<a href="reference.html#P8">[P8]</a>,<a href="reference.html#P8">[P9]</a>

<br>

    <dd><li>We contribute to society in health care by elucidating structural dynamics of disease-associated proteins. We have already elucidated functional mechanisms of dihydrofolate reductase, gene repaired protein (MutL) and etc. </li>

    </dd></dl>


    <dl><dt><font size="6">D</font>evelopment of new tool, software, and experiments for protein dynamics</dt><a href="reference.html#P1">[P1]</a>,<a href="reference.html#P4">[P4]</a>,<a href="reference.html#P7">[P7]</a>,<a href="reference.html#RJ1">[Rj1]</a>,<a href="reference.html#S1">[S1]</a><br>

    <dd><li>The application and the system refinement of H/D exchange using MALDI-TOF MS</li>

    <li>The development of site specific H/D exchange by double digestion using multi-peptidases</li>

    <li>The development of micro-fluidic device for millisecond quenching of H/D exchange</li>

　　<li><A href="http://homepage1.bb-west.ne.jp/pepdesi/ProteinDesignist.html">The software development for the fragment identification and analysis of post-translation modification (JAVA)</A></li>

<li><A href="http://homepage1.bb-west.ne.jp/pepdesi/XDscipas/XDscipas.html">The software development for the H/D exchange analysis of proteins using MALDI-MS coupled with pepsin digestion. (JAVA)</A></li>   

<li>The software development for the H/D exchange kinetics analysis of proteins using MALDI-MS coupled with pepsin digestion. (JAVA)</A>　etc.</li>   

    </dd></dl>

    </tr>

<tr><td>

<HR>

<a href="reference.html"><font size="4">Achievements.</font></a>

<HR>

<font size="4">Please access 「<IMG SRC="access.jpg" ALT="access">《 for this project.</font>

<HR>

<center><div class="cprt">Copyright (C) 2010- Tatsuya Yamamoto. All Rights Reserved.</div></center>

</td> </tr>

</BODY>

</HTML>